The job satisfaction of educators plays a crucial role in the quality of education and the overall performance of academic institutions. This study investigates the effect of work environment and organizational culture on the job satisfaction of basic science teachers in colleges and universities. Using a mixed-methods approach, we surveyed 300 basic science teachers across various higher education institutions and conducted in-depth interviews with a subset of 30 participants to gain comprehensive insights into their experiences. Our findings reveal a significant correlation between a positive work environment and high levels of job satisfaction among basic science teachers. Key factors contributing to a favourable work environment include adequate teaching resources, manageable workloads, supportive administrative policies, and opportunities for professional development. Additionally, the study highlights the critical role of organizational culture in shaping job satisfaction. An inclusive, collaborative, and transparent culture that values and recognizes teachers' contributions significantly enhances their job satisfaction. The implications of this research suggest that educational institutions should prioritize creating supportive work environments and fostering positive organizational cultures to enhance job satisfaction among basic science faculty. By addressing these aspects, institutions can improve teacher retention, job performance, and ultimately, student outcomes. Future research should explore longitudinal effects of these variables and consider comparative studies across different academic disciplines and regions.