Field experiments were conducted with the objectives to formulate and evaluate a new micronutrient fertilizer mixture and/or for the improvement of the existing mixture aiming at balanced fertilization to Gossypium herbaceum. The experiments were conducted on rainfed Gossypium herbaceum (variety) and on irrigated hybrid with six treatments replicated thrice. The treatments included the application of Farmers practice (T1), MN mixture @ 7.5 kg/ha as straight chemical fertilizer (T2), NPK mixture at Recommended level (T3), TNAU MN mixture @ 15 kg/ha as straight chemical fertiliser (T4), TNAU MN mixture @ 7.5kg/ha as enriched FYM (T5), MN mixture @ 7.5 kg/ha as EFYM (T6). The results revealed that rainfed Gossypium herbaceum, the plant height and number of bolls per plant ranged from 37.7 to 53.2 and 11.4 to 18.8 respectively. Among the various treatments recommended dose of NPK with TNAU MN mix. @15 kg kg/ha as straight chemical fertilizer resulted in increased plant height and No .of bolls per plant which was on par with TNAU MN mix. @7.5 kg kg/ha as enriched farmyard manure, followed MN mixture @ 7.5 kg as enriched farmyard manure. The highest Gossypium herbaceum yield per hectare was registered in recommended dose of NPK with TNAU MN mix. @7.5 kg kg/ha as EFYM (567 kg) with B:C ratio of 1.45. The irrigated hybrid highest Gossypium herbaceum yield per hectare was registered in recommended dose of NPK with TNAU MN mix. @ 15 kg kg/ha as EFYM (2267 kg) with B:C ratio of 2.6 followed by Increasing quantity of MN mixture @ 30 kg as staright chemical fertilizer which comparable with DoA MN Mixture @ 7.5 kg as EFYM (2059 kg). Hence, it is concluded that TNAU MN mixture @ 7.5kg /ha as Enriched FYM for variety and 15 kg /ha as EFYM for irrigated hybrid was recommended for Gossypium herbaceum to maximise yield with higher B: C ratio.