Hospital environment may serve as important reservoirs of multidrug resistant pathogenic bacteria, or they can also emerge in the healthcare facilities as a result of mutation or horizontal gene transfer. In the present study, we sequenced and phenotypically characterized, a Gram-negative, rod-shaped isolate recovered from infected orthopaedic implant samples collected from a hospital in Islamabad, Pakistan. The optimum growth temperature and pH was 37C, pH 7, respectively under laboratory conditions. Colonies on BHI and LB agar were round, smooth, raised and off-white in colour. The genome of the isolated strain denoted as MB649 was sequenced, the results showed 94% similarity to Enterobacteriaceae family, 85% to genus Enterobacter and 82% with specie cloacae. The genome analysis revealed presence of several secretion systems such as type II, IV, V, VI, VIII along with several heavy metal resistant genes such as systems for copper, nickel, tellurium, and tellurite resistance and multidrug resistance genes as well. The strain also encoded two plasmids IncHI1A(NDM-CIT) and IncHI1B(pNDM-CIT). Assembly resulted in 5297873 base pairs and 239462 reads distributed in 104 contigs, with a G + C content of 54.46 %. Sequence and annotation data of the E. cloacae MB649 genome have been deposited at GenBank under accession JABWTL000000000.