Milk production has an economic interest in the light of population explosion to meet the market requirements. The present study aimed to increase milk production using lysine as a food-fortified product. The study was conducted using 36 Awassi ewes. Ewes were divided randomly into three groups; each group was 12 ewes. The second group (T2) was presented with the standard diet of 4 gm of protected lysine, while the third group (T3) was presented with the standard diet of 8 gm of protected lysine, and (T1) represented the control group. The results indicated that there was a significant increase (P≤0.05) in the weights of ewes and their newborn lambs at the end of the study, but there are no significant changes in milk production, solid-to-fat ratio, milk density, protein level and lactose percentage among the transactions at the beginning of the experiment, except for a significant decrease (P≤0.05) in the percentage of milk fat in the second group. At the end of the experiment (the third month), a significant increase in the daily milk production, milk fat, solid to-fat ratio at 8 gm of protected lysine (T3) was observed in the third treatment, while no significant changes in milk density, protein level and lactose percentage happened among the transactions. As for the results of the hormonal analysis, the results indicated a significant increase (P≤0.05) in prolactin, growth and thyroxine hormones in the two groups treated with protected lysine accompanied by a significant decrease (P≤0.05) in cortisol levels in the treated groups compared to the control group.