The main objectives of the production of pistachio (Pistacia vera L.) transplants from a certified cultivar are to preserve the genetic characteristics of the plant species and to produce healthy, strong transplants with excellent genetic characteristics that are suitable for the environment and can withstand difficult climatic conditions in the appropriate places for them to be planted. Hence, the current study was carried out to produce P. vera transplants from a certified cultivar during the early stages of the nursery. The study was conducted during the growing season of 2021 in Bani village, Duhok city, in the Kurdistan Region of Iraq. The aim of this study was to investigate the effects of three pistachio cultivars (Abas-Ali, Kale-gnocchi, and Siirt), find out the best dates of budding among the three dates (med-June, 14 August, and med-September), and determine the best concentrations of Kinetin among the three cultivars (0, 5, and 10 mg/L) and their interactions. The T-budding methods were used on one-year-old transplants. The results show that the Siirt cultivar recorded the highest budding success percentage. At the same time, the Abas-Ali cultivar dominated the section height and length of lateral branches. Furthermore, the first budding date (med-June) recorded the maximum values of budding success percentage, section height, scion diameter, number of lateral branches, and length of lateral branches. On the other hand, the third concentration (10 mg/L) of Kinetin showed the highest values of budding success percentage, section height, and the number of lateral branches. However, the most effective interactions were cultivar Siirt, the first budding date, and concentration (10 mg/L) of Kinetin, which increased budding percentage and section diameter.